Atividade antibacteriana do extrato hidroalcoólico da Moringa oleífera sobre Pseudomonas aeruginosa.

Abstract

The Moringa oleiferous lam is a legume that can present wide antimicrobial property, as well as economic and medicinal value, providing improved health. From India, it is known as White Acacia in Brazil, where it has adapted well to the Northeast region. Oleferous Moringa and several other medicinal plant extracts have shown antimicrobial effects and, when associated with drugs, may represent a therapeutic alternative in the fight against infectious diseases such as Pseudomonas aeruginosa which is a non-fermenting bacillus with high resistance to antibiotics, capable of infecting animals, insects and humans. The objectives of this research are: to analyze the antibacterial activity of the hydroalcoholic extract of oleiferous Moringa (EMO) on the pseudomonas aeruginosa strain (ATCC27853), to determine the minimum inhibition concentration (MIC) of EMO and to verify the interaction of this extract associated with the antibiotics Benzylpenicillin, Ciprofloxacin and Oxacillin. After dilution, the EMO was placed in a 96-well plate to determine the minimum injunction concentration (MIC). The activity modulating the action of antibiotics was evaluated by the subibibian concentration of EMO and antibiotics at decreasing concentrations, from 1024μg/ml up to 16μg/ml. The test results showed that EMO does not have clinically relevant antimicrobial activity for Pseudomonas aeruginosa (ATCC 27853. We observed ciprofloxacin inhibition when associating EMO. In combination with Oxacillin, EMO demonstrated antibacterial activity in all wells, while with Benzylpenicillin it has not undergone changes. Due to the fact that the extract did not demonstrate antibacterial action on the Pseudomonas aeruginosa strain, and it is not possible to report the reasons, it is suggested to identify the chemical components of the oleferous Moringa seed, as they may vary due to seasonality, collection period, different geographical origins, presence of contaminants, among others. The main discovery of this research was the drug interaction of oleferous Moringa associated with the antibiotic Ciprofloxacin inhibiting its action on Pseudomonas aeruginosa, suggesting avoiding the consumption of this plant concomitantly with this drug until further research is carried out in the context of drug interactions for more details.