Desenvolvimento de um sistema eletrônico de aquisição e processamento de biosensores.

Abstract

This dissertation presents the description of the development of one Acquisition and Process Electronic System for Biosensores that use SPR principle. The proposed system offers one alternative totally portable to the current commercial equipments existent. The VIR bio-chip is used to compose the sensitive surface, offering maintenance advantages and easiness of change of the surface. The developed system uses one dual core processor OMAP1510 to accomplish the processing of the acquired sign, and to execute the user interface. One electronic circuit was developed to excite the SPR effect, to acquire the image sign, to control the laser current and temperature. An interface with the user was developed to offer analysis resources and processing of the obtained sign, beyond of allowing the control the instrument set points. This turns the system flexible and suitable to be used as research tool. The developed system was applied to design a new technique of BSA binding proteins analysis. Through the analysis of the morphology of the SPR curve, with the measurement of the resonance line broadening parameter and line asymmetry, it was shown that is possible to analyze reversible and irreversible BSA binding in the formation of the protecting layer.