Mapeamento e análise in silico das proteínas referentes ao estresse salino em espécies do gênero do algodoeiro ( Gossypium ssp.).

Abstract

Cotoniculture is a very important plantation in Brazil, holding the fifth place as the largest producer and the third largest exporter in the world. Nationally, the planting of herbaceous cotton is concentrated in the Midwest and Northeast, where it is mostly cultivated by irrigation, followed by the rainfed technique and without irrigation. Climatic factors, high evapotranspiration and low rainfall, irrigation water quality, management, soil and species characteristics, are determinant for the existence and aggravation of saline stress at planting, being one of the most important abiotic stresses, along with water, affect fiber growth, productivity and quality. For this, plant genetic improvement is the most viable alternative to reduce the consequences generated in the plant by excess salts, such as the use of chemical products in the crop and the quantitative and economic loss of production. With design of in silico primers that will recognize conserved domains in the gene related to tolerance and/or resistance, enabling genetic manipulation to create cultivars more adapted to this factor. Thus, from bioinformatics, the present work analyzed taxonomically and physico chemically three selected proteins of interest, according to previous screening, designing the primers, validating them and selecting the ten most stable primers, six from the proteins and four degenerates, the which are safe, specific and in accordance with qualitative reference standards. Of extreme relevance for the experimental area, the knowledge of the profile, action of proteins and design of molecular markers carried out, the way to minimize the problems generated by salinity in cultivation is palpable.