Estudo da ação inibitória de própolis associada à antibióticos comerciais sob cepas de Staphylococcus Aureus.

Abstract

Natural products have a wide therapeutic application because they present antimicrobial activity in their chemical composition against several microorganisms and because they are an effective alternative for the treatment of numerous infectious diseases. Several literatures have indicated that propolis has a great therapeutic potential, highlighting the activities, antioxidant, antimicrobial and anti-inflammatory. Red propolis is a resin of varied color collected by bees of the species Apis mellífera from various parts of the plants. The main origin comes from Dalbergia ecastophyllum, popularly known as howler tail, found along beaches and mangrove regions of northeastern Brazil. Staphylococcus aureus is a spherical bacterium of the gram-positive group, a pathogen responsible for a wide variety of clinical syndromes, with action ranging from the simplest to the most invasive infections. In this context, this study aimed to evaluate the antibacterial activity of the hydroalcoholic extract of red propolis on staphylococcus aureus strains associated with commercial antibiotics. The minimum inhibitory concentration was determined by the microdilution method in wells diluted sequentially by the title 1:2 and served to evaluate the substances as modulators of antibiotic resistance. Then, the modulating activity of the action of antibiotics was performed, using the subinhibitory concentration of the extract and antibiotics in decreasing concentrations, starting from 1024μg/mL, sequentially diluted up to 16μg/mL. The results of the tests showed that the extract of red propolis has clinically relevant antimicrobial activity for Staphylococcus aureus, because the MIC value was below 1024 μg/mL. Benzetacil antibiotics showed antibacterial activity against this strain, while Ciprofloxacin showed no action. No synergistic effect was observed when we associated the extract with the antibiotic Ciprofloxacin in modulation, a fact that became identical to MIC, because the fact may be related to binding receptors both in the drug and also in the bacteria tested, and to the chemical components of red propolis. However, propolis characterizes to be more profitable, contributing positively to ensure better results and to have control and/or decrease and costs in the treatment of Staphylococcus aureus infections.