Composição química, ação antibacteriana e moduladora da folha da cajazeira (Spondias mombin L.) sobre enterobactérias diarreiogênicas.

Abstract

Diarrhea is the occurrence of three or more soft or liquid bowel movements within 24 hours. Infectious agents are the most significant cause of acute diarrhea. With this, multiple plant extracts are being studied; among them, the species Spondias mombin L., popularly known as cajá, whose leaves have antimicrobial activity, deserves special mention. Thus, this study aims to report the in vitro expression of the hydroalcoholic extract of Spondias mombin L. (Cajá) on enterobacteria and irrigation agents. Methodologically, it is exploratory research, which follows an experimental technical procedure with a quantitative approach. It was performed in partnership with the campuses of Pombal and Cajazeiras of the Federal University of Campina Grande. The Cajá leaves were weighed, macerated, and immersed in a hydroalcoholic solution in a concentration of 1:1 for 72 hours. Then the solution was filtered, and the alcohol evaporated. The experiments were carried out with the standard Enterobacteria lineages from diarrheal lineages, all obtained from the collection of microorganisms from the Microbiology Laboratory of the Teacher Training Center (CFP / UFCG). The antibiotic, Benzetacil (B), Ciprofloxacin (C), and inoculum with the sample (A) were used to assess the modulating activity of enterobacteria (E. coli, Salmonella and Shigella). The data were expressed as mean values and ± standard error. The results showed that after 24hour period in a BioOxygen Demand (BOD) oven, it was observed through staining with Nimesulide that the antibiotics were effective against the Escherichia coli bacteria. However, the analyzed sample did not show potential for the control of the microorganism. The CIM / 8 was used in the next stage, composing inoculum, sample, and antibiotic. After 24 hours, the staining with Nimesulide was performed, demonstrating that the sample had an antagonist action with the antibiotic effectiveness, allowing the loss of the effectiveness of the medication.