Isolamento do toxoplasma gondii de galinhas (Gallus gallus domesticus) de criações domésticas em camundongos no Estado da Paraíba, Brasil.

Abstract

Isolation of Toxoplasma gondii from domestic chickens (Gallus gallus domesticus) in mice in the State of Paraíba, Brazil. The objective the research was to perform the isolation and watching the toxoplasma gondii virulence of HIV-positive domestic chickens in Swiss mice Of the total of 152 33 seropositive animals were subjected to bioassay, the bioassay for the brain and heart samples were used in the amount of 1.0 mL per animal subcutaneously. The titers of anti T. gondii antibodies ranged from 1:16 to 1: 4096, the most frequent 1:64 (44/152). The positive animals, 71 were submitted to the mouse bioassay for insulation, resulting in 33 isolates, of which 27 (81.8%) were lethal to at least one of inoculated mice, they came to death between 16 and 45 postinoculation (p. i,) acute toxoplasmosis confirmed by the presence of the parasite in the tissues of animals. 21 (63.6%) isolates were lethal to all inoculated and six (18.2%) isolates all mice survived up to 42 days p. i. whereupon it was carried out serology and positive were euthanized on day 60 p. i., with detection of the parasite in their tissues. It was observed that the percentage of isolation increased as antibody titers were higher, reaching 100% isolated in titers greater than 2048, the obtained results indicate environmental contamination with high virulence index indicating the possibility of human infection as well as animals of the region studied.