Efeito do sulfato de vincristina sobre epitélio intestinal e seminífero de ratos.

Abstract

Effect of Vincristine Sulfate on Intestinal Epithelium and Seminiferous rats. In order to evaluate the influence of the use of vincristine sulfate on rapid multiplication epithelia in this work are described morphology of the intestinal epithelium (duodenum, jejunum and ileum) and the seminiferous epithelium of rats treated with 15 days of life and euthanized in pubertal phase. Mostly the currently available chemotherapeutic agents act in the destruction of cells in intense multiplication, not exclusive presenting cell selectivity of tumor cells, causing many deleterious effects in both healthy tissues and in neoplastic cells. Among the most susceptible tissues are the seminiferous epithelium and intestinal epithelium since they are in constant cell replication. 10 animals (5 and 5 controls treated with vincristine sulfate) were used. The drug applications occurred at 15 days of life, and euthanasia at 64 days of life. body weights were measured, intestinal morphometric measurements (villus height and crypt profundida) and testicular morphometric measurements (tubular overall diameter, the diameter of the tubular lumen the height of the seminiferous epithelium). The biometric measurements were made on all animals in the experiment, intestinal morphometric evaluations were performed in 120 villi and crypts 120 per intestinal segment, and the morphometric testicular ratings in 200 seminiferous tubules. Thus chemotherapy can cause serious damage to the intestine and testis, in particular the height of the intestinal villi, reflecting directly on weight gain. It also promotes the reduction of the testes resulting in temporary or permanent infertility. However the use of the drug did not significantly influence cell renewal of intestinal mucosa, and thus the average body weight was also not changed.