Diagnóstico do calazar canino: tecido versus protocolo de extração de DNA. Qual a melhor opção?

Abstract

The purpose of this work was to evaluate the best source tissue and the most efficient DNA extraction method for the diagnosis of canine visceral leishmaniasis by PCR. Samples were taken from spleen, liver, popliteal lymph node, bone marrow, conjunctiva and peripheral blood of naturally infected dogs, from which 16 were asymptomatic and 2 symptomatic. DNA extraction was performed with commercial kits and isopropanol or sílica methods. The results suggest that the best association includes the extraction of DNA from popliteal lymph node using a kit. In the conditions described here, the PCR sensitivity reached 72% in a sample where 88% of the dogs were asymptomatics.