Diagnóstico molecular de Leptospira sp. com catetos (Pecari tajacu Linnaeus, 1758).

Abstract

Molecular diagnosis of Leptospira sp. with collared peccaries (Pecari tajacu Linnaeus, 1758). The collared peccary (Pecari tajacu) belongs to the order Artiodactyla, to the suborder Suiformes, superfamily Swine and to the family Tayassuidae and are recognized for their great capacity of physiological adaptation and the escape for their behavior and it shows with potential and zootechnical value for the production of meat and leather, being the company one of the most consumed wild in Brazil. There is a paucity of data available in the literature that infects catastrophes, such as leptospirosis, an emerging disease with high prevalence in tropical and subtropical countries. In addition, even if the user is unavoidable, stressors are not described categorized in the animal. Inspection of previous evidence in wild species for a certain management in the veterinary preventive practice to then introduce the animal into a commercial breeding. The objective of this study was to detect Leptospira sp. DNA, using samples from the reproductive tract of collared peccary. The methodological systems were used in conjunction with MMA, ICMBio and SISBio with protocol number 36263-5. It was approved by the Ethics Committee on Animal Use Research (CEUA / CSTR / UFCG), Patos campus with the protocol number 05 / 2018. As the field activities include a collection of vaginal and preputial secretion in swine from all the hounds from breeding sites then the DNA was extracted by using two primers LipL32 -45F and LipL32-286R with amplification of the LipL32 gene as a target. In the same 48 vaginal fluid samples were performed PCR and all animals were negative in the assay. No presence of the etiological agent was evidenced by the molecular diagnosis, however it was not possible to disregard the participation of the same as reservoirs in the infections. Since it is a zoonotic of a reproductive and global risk character, together with a series of research opportunities, research is needed on the outbreaks of infectious diseases in wildlife, in free-living animals and captive animals. In the same way, evaluated, epidemiological, in the maintenance of animal health, to reduce losses and ensure a state of conservation.