Análise in vitro do grau de toxicidade do extrato etanólico de Azadiracta indica A. Juss em Artemia salina e em larvas infectantes de Haemonchus contortus.

Abstract

In vitro analysis of the degree of toxicity of the ethanolic extract of Azadiracta indica A. Juss in Artemia salina and in infective larvae of Haemonchus contortus. Gastrointestinal parasites, especially Haemonchus contortus cause reduction in livestock productivity of small ruminants. The lack of knowledge of creators and inadequate application of anthelmintics generates parasitic resistance. in search new methods to reduce the use of chemical products, herbal medicine is a new Alternatively, among the plants, there is the Azadiracta species indicates, known as Nim, who owns repellent and anti - helminthic. For toxicological studies plants, microcrustacean Artemia is widely used to replace the use of guinea pigs in experiments. Initially, the extract was prepared from the leaves of Neem subsequently microcrustacean Artemia salina was reproduced in the laboratory, to evaluate the toxicity of the hydroalcoholic extract of this plant. The test was conducted in triplicate, with 10 specimens of A. saline on each plate containing the extract in at concentrations of 100, 500, 1000, 10.000 and 20.000 mg / mL, after 24 hours was done to count the nauplii survivors. For the in vitro test for H. contortus extract in concentration of 18.000 mg / ml was added and the stool cultures after seven days proceeded to read, to count of third-stage larvae (L3). The degree of toxicity of the hydroalcoholic extract of the leaves of neem on A. Saline is low, but when tested in Haemonchus contortus the result was satisfactory. Thus, A. indicates represents a viable alternative for the control of parasitic infections in small ruminants.