Constituição química e avaliação da atividade do óleo essencial de Croton argyroglossum Baill em bactérias constituintes do biofilme dental.

Abstract

Natural compounds have been used on oral biofilm inhibition in order to solve antimicrobial resistance, improve pharmacological action, and decrease toxicity and costs. The aim of this study was to evaluate the efficiency of extraction of essential oil from Croton argyroglossum Baill, physicochemical properties, composition of the chemical constituents and evaluate "in vitro" antibacterial activity against the major bacterial biofilm strains - Streptococcus mutans, Streptococcus sanguinis and Streptococcus sobrinus. The essential oil extraction was performed using Clevenger-type apparatus. Physicochemical analysis – relative density (picnômetro, 20° C), refractive index (Abbé Refractometer, 20° C) solubility on ethanol at 90%, color and appearance (visual analysis). The chemical constituents were identified and quantified by gas chromatography attached to a mass spectrometer (GC-MS). Evaluation of in vitro antibacterial activity of the essential oil was held by the performed in diffusion plates. The yield was obtained from 0.93%. The essential oil were observed: relative density- 0.9091; refractive index- 1.4850; solubility on ethanol- 1:1; color - light yellow and appearance - clear. The gas chromatography attached to a mass spectrometer analysis identified 57 constituents. The major compounds found in the oil were eucalyptol (15.59%), bicyclogermacrene (13.91%), sabinene (13.09%) and α-pinene (6.52%). The in vitro evaluation of essential oil antibacterial activity, performed in diffusion plates, formed inhibition zones lower than 10 mm, indicating the resistance of the analyzed strains.