Avaliação da atividade antibacteriana do monoterpeno (S)-(-)-Citronelal contra cepas de Pseudomonas aerunginosa.

Abstract

Evaluation of the antibacterial activity of the monoterpene (S)-(-)-Citronellal against Pseudomonas aerunginosa strains. The use of natural products in dentistry constitutes a viable and effective alternative in the prevention and combat of various pathologies of the oral cavity, highlighting the O and its enantiomers belonging to the group of Monoterpenes, derived from the metabolism Secondary plants. This compound already Gisele several biological Activities tested and confirmed such as antimicrobial. The present study aims to evaluate the antibacterial activity of the monoterpene (S)-(-)-Citronelal against Pseudomonas aeruginosa strains. In the methodology, the serial microdilution technique was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). In a 96-hole plate, Mueller Hinton broth was added, the monoterpene in the different concentrations and the inocúlo of the bacteria strains. The plates were incubated at 37 º C for 24-48 hours. After reading the MIC, aliquots of 20 μL were removed from each well that did not present bacterial growth, and transferred to Wells of a new plaque, devoid of any antimicrobial, was aseptic closed and incubated at 35 °c, and the CBMs were Recorded after 48 hours. It was also performed the minimum adhesion inhibitory concentration (MICA), through a simulation, in vitro, of the dental biofilm. The results obtained for the monoterpene tested were MIC equal to 512 μg/mL, thus presenting a strong antibacterial effect, but not presented MBC values and proved effective in inhibiting adherence of Pseudomonas aeruginosa to a concentration of 1:8. Based on the results it is concluded that the Monoterpene (S)-(-)-Citronelal has an effective bacteriostatic and non-adherent effect on the strains of Pseudomonas aeruginosa.