THEODORO, P. I. L.; http://lattes.cnpq.br/0390864647085192; THEODORO, Paulo Itagino Lopes.
Résumé:
Considering the pharmaceutical industry's need new substances that can give rise to new drugs,
aiming to be more effective than the current ones and to have the ease of reproducibility of
results, this work aims to analyze the production of secondary metabolites of endophytic fungi.
from Aloe vera. Endophytic fungi inhabit the internal tissues of plant species and have great
interaction with the host, such interaction can give them the ability to produce the same
chemical substances originally characterized by the host plant, which is beneficial of
pharmaceutical industry as it already has well-established techniques. large-scale production of
substances from fungi. The choice of the plant species Aloe vera is justified by its
ethnobotanical indication, which shows its soothing, moisturizing, healing and anticancer
properties, among others. The plant parts were collected in the nursery on the campus of the
Centro de Desenvolvimento Sustentável do Semiárido. Subsequently, the endophytic fungi
were isolated in Potato-Dextrose-Agar medium containing Azithromycin. The fungi were
cultivated for conidia production and then were re-cultured in oat medium, inducing the
production of secondary metabolites. In turn, the different extracts obtained were analyzed
using High Performance Liquid Chromatography technology, using a Reverse Phase column
(HPLC-FR), resulting in 94.28% of the 35 secondary metabolites found having a hydrophobic
molecular structure that, according to the literature, have a large antifungal and antiparasitic
potential, supporting positive conclusions for possible obtaining substances with potential for
the origin of new drugs.
