Utilização de diversas soluções hiposmóticas, na avaliação de sêmen ovino refrigerado diluído em água de coco em pó e leite em pó desnatado.

Abstract

This work aimed to study the effectiveness of different solutions using hyposmotic semen from two rams of the Santa Ines breed, diluted in water, coco powder and milk powder and cooled to 5 °C. The solutions were made hyposmotic sodium citrate and fructose, at concentrations of 150 mOsmol/L and 300 mOsmol/L. Ten samples were made by breeding and semen immediately evaluated and submitted to the hyposmotic test periods of 12, 24 and 48 hours after dilution. The results it was found in vitro sperm viability after dilution using the two solvents, and their conservation satisfactory to 5 °C in the first 24 h. Concluding that the hyposmotic test was effective in evaluating the plasma membrane of spermatozoa in different periods.