Utilização do teste hiposmótico na avaliação do sêmen refrigerados de pequenos ruminantes.

Abstract

The present work had as objective to evaluate the use of different solutions hypoosmotic to perform the hypoosmotic test (HOST) in assessing the semen goat three reproducers animals of Moxotó breed, diluted in coconut water powder and kept under refrigeration at 5oC. Two sodium citrate and fructose based solutions were used with osmolarities of 150 and 300 mOsmol/L and a solution of distilled water. Four semen collected were harvests in each reproducer, being evaluated against macroscopic and microscopic characteristcs and submitted to the test HOST after 12, 24 and 48 hours postrefrigeration. The results showed that the individual progressive motility and vigor have not suffered significant differences up to 24 hours post-refrigeration. There were no changes between the different osmolarity hypoomotic solutions in assessing the integrity of goat semen sperm membrane.