Mastite subclínica em cabras no Estado da Paraíba: ocorrência, etiologia, susceptibilidade antimicrobiana e fatores de risco.

Abstract

An epidemiological survey to caprine subclinical mastitis in the Paraíba State was conducted to determine the occurrence of the infection, to isolate and to identify the involved microorganisms, as well as to evaluate the antimicrobial sensitivity of the isolated agents and to determine the agreement between California Mastitis Test (CMT) and bacterial isolation. Sixty nine goat milk samples from seven herds were collected. During milk collection the physical examination of mammary glands and the CMT were carried out. Milk samples were cultured on 5% sheep blood agar and incubated at 37 oC under aerobic conditions for 48 hours, and colonies were identified by Gram staining method and biochemical tests. The sensitivity of the coagulase-negative Staphylococcus (CNS) samples was tested to antimicrobials gentamicin 10 mcg, florfenicol 30 mcg, kanamycin 30mcg, amoxicillin 10 mcg, enrofloxacin 5 mcg, cefquinome 30 mcg e NBT (neomycin 30 mcg, bacitracin 10mcg and tetraciclin 30 mcg). Of the 69 analyzed milk samples, 29 (42%) presented bacterial growth. The CNS were obtained in 81% of the isolated ones. Thirty six samples (52.2%) were positive at CMT, which presented slight agreement compared to bacterial cultive, as well as low sensitivity and specificity, 55.17% and 50% respectively. Kanamycin, gentamicin, cefquinome, florfenicol and NBT presented anticrobial sensitivity upper to 90%.