Utilização de fosfato de cálcio, quitosana e medula óssea no reparo ósseo em coelhos, estudo clínico-cirúrgico, radiológico e histológico.

Abstract

The aim of this study was to compare the use of different calcium phosphates in bone repair in rabbits. Twenty rabbits of both sexes, New Zealand breed, weighing in average 2.5 ± 0.41 kg were used. Animals were randomly divided according with two experimental moments, being 10 animals euthanized 30 days after surgery, and 10 euthanized 60 days after surgery. Four implants of calcium phosphate which constituted the groups were used. Materials were introduced into the proximal and distal metaphysis of each femur so that CPP 700 and 900 were introduced into the left limb, while TCP/HA and HA in the right limb. Clinical-surgical assessments were performed daily, and radiographic assessments were made before and after surgery and at 30 and 60 days post-operatively. All animals showed normal clinical progression. At the radiographic assessments statistical difference (p <0.05) was observed between groups, with higher radiopacity in lesions filled by TCP/HA and HA implants. At the histological evaluation by light microscopy and morphometric analysis, materials TCP/ HA and HA showed better bone formation around the implants than CPP700 and CPP900, and that was also observed at 30 and 60 days of evaluation.