Pesquisa de Mycobacterium avium subsp. paratuberculosis em caprinos e ovinos provenientes de matadouro na Paraíba.

Abstract

Paratuberculosis is an infectious disease, chronic, incurable that affects several species of animals, causing a chronic granulomatous enteritis and lymphadenitis, characterized by malabsorption syndrome, diarrhea and weight loss, reduced productivity in influencing and causing great impact on the economy. Caused by Mycobacterium avium (subsp.) paratuberculosis (Map), which is also considered as the causative agent of Crohn's disease in humans. This dissertation consists of two original articles. The first submitted to the journal Archives of Biology Institute which is an explanation of paratuberculosis in Brazil with a focus on sheep and goats; definition, etiology, pathogenesis, clinical signs of these particular species, gross and microscopic lesions, diagnosis and risk factors associated with prevention the disease. The second chapter was submitted to the journal Veterinary Research addressing the occurrence of the Map in goats and sheep from abattoirs in Paraíba. Where samples of mesenteric lymph nodes and ileocecal valves were collected from 151 animals (77 sheep and 74 goats) for the diagnosis by histopathology using hematoxylin and eosin staining and Ziehl-Neelsen, and the Polymerase Chain Reaction (PCR). In histopathology by H & E staining, there were no nonspecific inflammatory lesions consistent with paratuberculosis. There were visualized acidfast bacilli in resistant samples, however, by the PCR technique, 15.6% studied in sheep and goats 12.16% were positive for Map.