Utilização de bactérias produtoras de dehalogenases no controle da intoxicação por monofluoroacetato de sódio presente em Amorimia septentrionalis.

Abstract

Poisoning of domestic animals by plants containing sodium monofluoroacetate, cause great economic losses, since there is no antidote capable of reversing intoxication and prophylactic measures available are not totally effective. The ruminal detoxification is a mechanism that has been used as an alternative control in poisoning by plants. Can be realized by bacteria able to degrade toxic compounds, such as sodium monofluoroacetate, usually this degradation is facilitated by the production of enzymes called dehalogenases. The objective of this study was to evaluate, in goats, the resistance present in MFA Amorimia septentrionalis induced by inoculation of ruminal bacteria Pigmentiphaga kullae and Ancylobacter dichloromethanicus isolated from the rumen of goats. The goats were divided into two groups, each with 6 animals. In group 1, 60 ml of a solution containing the bacteria was inoculated into each animal daily for 10 days. In group 2, the goats received no bacteria. On the tenth day of inoculation was offered to all goats plant at a dose of 5 g/kg body weight daily in each animal being stopped watching after the first clinical signs of intoxication. Goats from group 1 showed clinical signs 5.83 ± 2.56 days after administration of the plant which differed significantly (p = 0.037) in goats of group 2, which showed clinical signs to 2.67 ± 0.52 days. The amount ingested by plant inoculated goats (28.83 ± 12.97 mg / kg) and noninoculated (12.03 ± 3.65 g / kg) to cause clinical signs was also statistically different between groups (p = 0.025). So it is concluded that administration of rumen bacteria Pigmentiphaga kullae and Ancylobacter dichloromethanicus induces resistance to poisoning plants containing sodium monofluoroacetate, enhancing its use as a means of controlling the poisoning.