Avaliação do perfil imunogênico de vacinas inativadas contra agalaxia contagiosa.

Abstract

The aim of this study was to evaluate the response of antibodies to surface proteins of M. agalactiae in sheep and goats vaccinated with inactivated vaccine consists of total antigen of M. agalactiae isolated in Brazil. Vaccine 1 antigen adsorbed onto aluminum hydroxide; Vaccine 2 antigen adsorbed onto Montanide IMSVG2215, and Vaccine 3 antigen adsorbed onto Montanide Gel01. The pool of sera from ten goats and ten sheep collected in periods 0, 21, 35, 90, 150, 210, 270 and 360 days after the first vaccination were tested by Western blotting techniques. In goats, vaccines 1 and 2 showed better results. The antibodies were observed 21 days after the first dose for all three vaccines. Regarding the profile of proteins in vaccine 1 stimulated the production of antibodies against proteins P48 and P55 and the vaccine 2 against P48 and P80. In sheep, the vaccine 2 was more antigenic with antibodies 21 days after the first dose. For both vaccines 1 and 3, antibodies were present 35 days after first dose, with a significant drop at 90 days, only antibodies against P48 discreetly appeared after the third dose. Stimulation induced by vaccine 2 produced antibodies against P48, P55 and P80 proteins that persisted until 90, 150 and 210 days after first dose, which rose again after third dose. These results reinforce the possibility of control of contagious agalactia using vaccination and suggest the possible use of P48 protein in diagnostic tests and vaccines in Brazil.