Parvovirose canina no Semiárido Paraibano: caracterização molecular, clínico-patológico e cardiovascular.

Abstract

The objective of this research was to identify the strain of canine parvovirus (CPV) isolates from feces of dogs with gastroenteritis in the city of Patos, Paraíba, Brazil. For this purpose, fecal samples from dogs were used aged between two to six months of age, without distinction as to gender and race. The extraction of DNA samples was performed by a commercial kit based on silica and amplified by PCR, a fragment of 583 base pairs of the VP2 gene. The amplification products were purified, sequenced, aligned using MEGA version 4.1 package and submitted to GenBank. The analyzes revealed that all samples were positive for CPV-2 and belonging to subtype 2b, which can be implicated as the variant responsible for developing gastroenteric disorders in young dogs in the city of Patos, in the state of Paraíba. These studies raise the importance of phylogenetic identification of strains of canine parvovirus in other areas of the Northeast, given the wide variability of biogeographical Brazil.