FERREIRA, D. H.; http://lattes.cnpq.br/0602208322622722; FERREIRA, Daneelly Henrique.
Resumo:
Antimicrobial resistance of Staphylococcus spp. and the identification of genes of
enterotoxigenic S. aureus mastitis in goats, the milk becomes subject to dissemination
of potentially pathogenic strains for consumers, assuming outstanding importance in
public health as the state of Paraíba stands with large dairy goats in Brazil. Thus, this
study aimed to evaluate the antimicrobial resistance of Staphylococcus spp. isolated
from goat mastitis and identify genes producing enterotoxins S. aureus. Both works
were sent to the Brazilian Archives of Veterinary Medicine and Animal Science.
Species identification and determination of minimum inhibitory concentration (MIC) of
antimicrobials were performed by using microdilution plates using semi automated
equipment (Autoscan4 ®, Siemens) based on colorimetric panels, basing on criteria
defined by the CLSI (2012). We identified 55 S. aureus, 56 coagulase positive
Staphylococcus (CPS) and 162 coagulase-negative Staphylococcus (CNS). The highest
rates of resistance were observed for ampicillin (100%) and penicillin (42%) and lowest
for nitrofurantoin (4%), trimethoprim / sulfametaxozol (8.8%) and ceftriaxone (8.8%).
As the production of β-lactamase, 131 (48%) strains were producing and 77 (28%)
showed multiple resistance to four or more antibiotics. The strains of S. aureus were
analyzed for the production of enterotoxigenic genes by PCR (Polymerase chain
reaction in) individual. It was found that 19.5% of the strains were positive for the
presence of genes and enterotoxigenic two genotypes were identified sec (16.7%) and
sei (2.8%). There was no association of two or more genes in the same strain.