Caracterização de Staphylococcus spp. isolados de mastite caprina.

Abstract

Antimicrobial resistance of Staphylococcus spp. and the identification of genes of enterotoxigenic S. aureus mastitis in goats, the milk becomes subject to dissemination of potentially pathogenic strains for consumers, assuming outstanding importance in public health as the state of Paraíba stands with large dairy goats in Brazil. Thus, this study aimed to evaluate the antimicrobial resistance of Staphylococcus spp. isolated from goat mastitis and identify genes producing enterotoxins S. aureus. Both works were sent to the Brazilian Archives of Veterinary Medicine and Animal Science. Species identification and determination of minimum inhibitory concentration (MIC) of antimicrobials were performed by using microdilution plates using semi automated equipment (Autoscan4 ®, Siemens) based on colorimetric panels, basing on criteria defined by the CLSI (2012). We identified 55 S. aureus, 56 coagulase positive Staphylococcus (CPS) and 162 coagulase-negative Staphylococcus (CNS). The highest rates of resistance were observed for ampicillin (100%) and penicillin (42%) and lowest for nitrofurantoin (4%), trimethoprim / sulfametaxozol (8.8%) and ceftriaxone (8.8%). As the production of β-lactamase, 131 (48%) strains were producing and 77 (28%) showed multiple resistance to four or more antibiotics. The strains of S. aureus were analyzed for the production of enterotoxigenic genes by PCR (Polymerase chain reaction in) individual. It was found that 19.5% of the strains were positive for the presence of genes and enterotoxigenic two genotypes were identified sec (16.7%) and sei (2.8%). There was no association of two or more genes in the same strain.