SILVA, F. F.; http://lattes.cnpq.br/9758187782108988; SILVA, Francisca Flávia da.
Resumen:
In Chapter I, the objective of this study was to evaluate the action of Monepantel and other
anthelmintics on gastrointestinal nematodes of sheep of the Paraíba Semi-arid. The second is
the influence of the three-year storage time of M. thaumasium pellets on the predation of
infective larvae of tricostrongilide sheep. The first study was developed in 20 properties, where
we used 30 sheep from each farm without anthelmintic treatment for at least three months. The
animals were divided into five groups: group I, treated with 2.0 mL / 10 kg of 10% Albendazole;
group II, treated with 2.5 ml / 10 kg Ivermectin 0.08%; Group III treated with 1.0 mL / 10 kg
of 10% Closantel; Group IV, treated with 1.0 mL / 10 kg of 5% Levamisole Hydrochloride; and
group V treated with 1.0 mL / 10 kg of 2.5% Monepantel. Fecal samples were collected at days
zero and ten to perform fecal analyzes. To evaluate the resistance, the reduction test was applied
in egg count per gram of feces (RCOF) and coproculture. Multiresistance was observed in all
the properties evaluated, where 95% of the farms had high resistance to Albendazole, 85% to
Ivermectin, 80% to Closantel, 40% to Levamisole and 45% to Monepantel. The second TRCOF
for Monepantel was performed four months after the first one at property 15, where the
vermifuge was ineffective, resulting in 75% efficacy. Two sheep were euthanized and
necropsied, and Haemonchus contortus, Trichostrongylus axei, Trichostrongylus
colubriformis, Oesophagostomum columbianum and Trichuris ovis were recovered. The second
study was carried out at the Federal Institute of Paraíba, Sousa campus. Eight negative OPG
sheep were divided into four experimental groups: Group I, 3g / 10kg of pellets of M.
thaumasium - 36 months of storage, dose only; Group II, 3g / 10 kg of M. thaumasium pellets
- freshly produced, single dose; Group III, 3g / 10 kg p.v, of pellets without fungi; Group IV,
control. Every 24 hours, up to 120 h, the faeces of the animals were collected and sent to the
Veterinary Parasitology Laboratory for analysis. Fifteen grams of feces from each animal were
weighed and five grams of expanded vermiculite were added, the coprocultures were added and
1000 infective larvae (L3) of tricostrongilídeos of ovinos, being the larval recovery realized
after seven days. It was observed that the predation of larvae in Group I (M. thaumasium - 36
months) did not differ statistically (p> 0.01) from Group II (M. thaumasium - recent),
respectively presenting reductions of 75 and 79%. Both groups added peak predation to larvae
at 72 h. The genus of helminth most recovered in coprocultures was Haemonchus sp. It was
concluded that the gastrointestinal nematodes of sheep are resistant to monepantel and that the
occurrence of multi-resistance to anthelmintic by these nematodes was frequent. It was
concluded that the 3-year storage period of M. thaumasium in the alginate matrix did not
influence the efficacy of predation of sheep trichotrongilide larvae with high reduction of larval
recovery and presence of fungal activity in the feces up to 96 hours after administration animals.