Desenvolvimento e padronização de ELISA indireto para diagnóstico da agalaxia contagiosa em ovinos e caprinos.

Abstract

This paper had as objective to present up to date about to distribution of the contagious agalactia of sheep and goats by culture and to carry on an indirect ELISA for diagnosis of this disease. Mycoplasma agalactiae was identified in 19 (9,35%) of the 234 goat and sheep samples which were proceeded from herds of Paraiba, Rio Grande do Norte and Pernambuco States. The ELISA-G was produced with whole and sonicated antigen from M. agalactiae isolated from a goat infected naturally. The standardization of ELISA was made using serum samples of free herds and infected animals, confirmed by culture. ELISA presented good capacity of discrimination between positives and negatives samples, with reason P/N of 46,2 and 42,8% when sonicated and whole antigen were diluted 1/400, respectively. Sensitivity, specificity and validation of the test should be determined from analysis of a bigger amount of samples.