TELES, J. A. A.; http://lattes.cnpq.br/1124304514859373; TELES, José Andreey Almeida.
Résumé:
This paper had as objective to present up to date about to distribution of the contagious
agalactia of sheep and goats by culture and to carry on an indirect ELISA for diagnosis of this
disease. Mycoplasma agalactiae was identified in 19 (9,35%) of the 234 goat and sheep
samples which were proceeded from herds of Paraiba, Rio Grande do Norte and Pernambuco
States. The ELISA-G was produced with whole and sonicated antigen from M. agalactiae
isolated from a goat infected naturally. The standardization of ELISA was made using serum
samples of free herds and infected animals, confirmed by culture. ELISA presented good
capacity of discrimination between positives and negatives samples, with reason P/N of 46,2
and 42,8% when sonicated and whole antigen were diluted 1/400, respectively. Sensitivity,
specificity and validation of the test should be determined from analysis of a bigger amount of
samples.