SANTOS, F. A.; http://lattes.cnpq.br/0135207299514378; SANTOS, Fabrine Alexandre dos.
Resumo:
This thesis is composed by three Chapters. In Chapters I, the study was conducted
experimental infection in two goats (animals 1 and 2) and two sheep (animals 3 and 4) with
an Actinobacillus seminis strain isolated from goat in Brazil. Samples of semen, puncture
and fragments of epididymis, deferent duct, testicles and seminal vesicles were used, and
histopathological, microbiological culture and molecular diagnoses were performed. At
clinical evaluation it were found unilateral swelling of firm consistency after 30 days in
epididymis and testicle from animal 4 that continued until the day of euthanasia, as well as
animal 1 shown discrete unilateral swelling of testicles. Gross and microscopic lesions in
animals 3 and 4 were compatibles with that caused by A. seminis infection. A. seminis was
isolated from material of puncture, testicle fragment and semen of one sheep (animal 4). In
Chapter II, the objective of experimentally evaluate the pathogenicity of an isolate named
SAAS01 in 15 goats. The animals were clinically analyzed over a 12-week period, during
which time semen collections were performed by electroejaculation, and the scrotal diameter
was measured every 20 days. These animals were challenged with 2 mL of a suspension
containing 1.2 x 10⁹ CFU/mL of A. seminis (SAAS01 isolate)16 by the following routes:
intrapreputial, cauda epididymis, and conjunctival. In the clinical evaluation, a unilateral
increase in firm consistency can be observed in the epididymides and testes of animals 18,
51, and 57 after 30 days; this firmness continued until the day of euthanasia. Animals 51 and
57 presented histopathological findings with macroscopically and microscopically
significant changes. The isolation of small (1 to 2 mm), smooth, shiny, and pigment-free
colonies was possible from the semen material collected from animals 18, 51, and 57 in the
third and fourth collections. DNA of A. seminis was amplified from the semen of animals
00, 12, 18, 22, 51 and 57, and from testicular fragments from animals 12 and 57. In Chapter
III, the objective of this work was to select samples from seropositive sheep in the AGIDA
test, both sexes, and from several states of the Northeast (PI, CE, SE, PB, RN), of which 130
were from Embrapa Goat and Sheep serum bank (N = 2465 sheep) and 41 from the sera bank
of the Laboratory of Communicable Diseases of the CSTR / UFCG (n = 1134 sheep), totaling
171 positive sheep, and compared with the Complement Fixation test (FC) and competition
ELISA. In the FC test, no positive serology was observed, while in the ELISA-i four animals
(2.33%) were seropositive and 20 animals (11.69%) suspected.