Avaliação da atividade antifúngica da fase acetato de etila da Rhaphiodon echinus contra cepas do gênero Candida spp.

Abstract

Candidiasis is a disease commonly diagnosed in the oral cavity of patients using total prosthesis. This is a fungal infection caused by yeasts of the genus Candida. Several antifungal agents are used for the treatment of this infection, however, therapeutic limitations, low efficacy and high toxicity are important factors for the search for a viable alternative for the treatment of this infection. Due to the clinical importance of oral candidoses, it is essential to investigate new therapies. Faced with this need, research with medicinal plants such as Rhaphiodon echinus has been promising. In this context, the objective of this work was to investigate the possible antifungal activity of the ethyl acetate phase of Rhaphiodon echinus on strains of Candida Albicans, Candida Krusei and Candida tropicallis by determining the minimum inhibitory concentration (MIC) on broth microdilution technique. For this purpose, 100 μl of the doubly concentrated liquid Sabouraud dextrose broth medium was used, then 100 μl of the Rhaphiodon echinus phase at the initial concentration of 2048 μg / ml dispensed in the first row of the plate and by serial dilution in two ratios the concentrations of 1024 to 4 μg / mL and last 10 μl of the inoculum of the fungal strains. As a result, MICs ranged from 256 μg / mL to 1024 μg / mL, as it obtained a 50% MIC of 512 μg / mL for all strains, so this product has high effectiveness to inhibit fungal growth, however research is needed to know what its mechanism of action is.