Ação antimicrobiana de carvacrol nanocarreado.

Abstract

Different natural compounds have been researched in recent years to be used as bactericidal or bacteriostatic agents under pathogenic or deteriorating bacteria in food. Among these compounds stands out the carvacrol. Carvacrol is a phenolic compound present in many essential oils such as oregano, thyme and marjoram. Staphylococcus aureus and Escherichia coli stand out among the different food-related microorganisms. The most common treatments for bovine mastitis are in this way, natural antibiotics, such as carvacrol, may be an alternative for the control of these microorganisms in food. Numerous articles are reported in the literature on possible microbial inactivations from carvacrol; however, their low affinity for water becomes a barrier to their use in food, since most of these matrices are water. Thus, in order to minimize these negative aspects, Bovine Sodium Albumin (BSA) based nanocarriers were formulated to carry carvacrol at different pH values (4,0,5,5 and 7,2) and evaluated their antimicrobial action by the Minimal Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (CMB) and the agar diffusion test. The formulations with the nanocarrelated carvacrol presented the same microbial dilution technique for the isolates (S. aureus and E. coli) of 0.50 μL. mL-1 for BSA-E at pH 4.0 and 1.00 μL. mL-1 at pH 5.5 and 7.2. For the formulations with caravacrol without nanocarrier they presented CMB of 1.00 μL. mL-1 at pH 4.0 and 2.00μL. mL-1 at pH 5.5 and 7.2. Analysis of the antimicrobial action spectrum by the agar diffusion technique (inhibition halo) demonstrated that the nanocarrier formulations were equally effective for Gram positive and Gram negative bacteria (p> 0.05). Thus, it was possible to observe that the nanocarreted carvacrol had an antimicrobial action more effective than carvacrol without being nanocarred, and could be a viable alternative as disinfectant for different segments in the agroindustry.