SILVA, P. V.; http://lattes.cnpq.br/0681185833020971; SILVA, Pedro Victor da.
Resumo:
Sweet potato (Ipomoea batatas) is a vegetable of national importance, also it is the fourth most
consumed vegetable in Brazil with great adaptation to different climates and soils and has a low
production cost. Nationally, the south and northeast regions have the title of being responsible
for the biggest part of the country's production, but currently the crops have been suffering
several damages with the infection of fungal pathogens, especially the species Fusarium
Oxysporum. The present work is intended to make a genetic study of the species Fusarium
Oxysporum f. sp. rapae, that belongs to the Fusarium Oxysporum genetic complex, and also
the production of primers for molecular diagnosis with the LYS2 gene. Researching genetic
sequences using the blast tool of the NCBI platform, 15 sequences were selected to form the
phylogenetic tree using the selected sequences and then design the primers for qPCR and the
primers specific of the LAMP technique. The results obtained from the phylogenetic tree, with
the bootstrap inference method, brought forward good results and similarities between species,
resulting in a good tree of species with representation of the historical topology of the species.
The primers designed with the primer3Plusplatform proved to be good primers, achieving all
the parameters and only three of the five primer sets designed were selected for the LAMP
technique. The developed protocols (in sílico) were based on molecular techniques to diagnose
F. oxysporum in sweet potato, one protocol for qPCR technique and another protocol for LAMP
technique.