Potencial das propriedades antioxidantes e anti-inflamatórias dos extratos de Turneasubulata (chanana) comprado a Harpagophytum procumbens (garra do diabo).

Abstract

Oxidative stress represents an imbalance between the production of reactive species and the biological system in its ability to readily detoxify reactive oxygen/nitrogen intermediates or repair the resulting damage. the components of the cell, including proteins, lipids and DNA. Each cell is characterized by a particular concentration of electrons stored in many cellular constituents, and the redox state of a cell determines cellular functioning. Disturbances in the normal redox state of tissues can cause toxic effects through the production of peroxides and free radicals that damage all components of the cell, including proteins, lipids and DNA. Thus, oxidative stress is an inevitable consequence of life in an oxygen-rich environment. Cyanobacteria are microscopic organisms widely distributed throughout the planet, as well as presenting relevant densities in continental water bodies destined for human activities. Such microorganisms are capable of producing secondary metabolites toxic to humans and other animals. It's cyanotoxins. When it comes to cyanotoxins, we can highlight the most common ones with the greatest toxic potential, which is the case of microcystins. Several studies report its ability to cause cellular oxidative stress in aquatic vertebrates after exposure to blooms of toxic cyanobacterial species, in addition to inflammation triggering various types of pathologies. In fact, studies demonstrate that toxic cyanobacteria systematically increase lipid peroxidation levels, which indicates the important role of oxidative damage and cyanobacterial toxicity. Plant extracts such as the plants Turnera subulata and Harpagophytum procumbens are widely applied in folk medicine, the extract from the leaves of Turnera subulata, a plant native to northeastern Brazil, for example, is applied as an alternative in the treatment of several diseases that have stress in common. oxidative stress and activation of the inflammatory system. However, despite its wide use, the effects of this compound are still not well described. However, the effects of Harpagophytum procumbens on these processes are now more clearly elucidated. The present study aimed to evaluate the levels of phenolics, flavonoids and anthocyanins to verify the antioxidant capacity and anti-inflammatory activity (in vitro) of Turnera subulata plant samples compared to Harpagophytum procumbens. Thus, we sought to evaluate the antioxidant and anti-inflammatory capacity of extracts from the leaves of Turnera subulata and roots of Harpagophytum procumbens, in an inflammation model (in vitro), through albumin denaturation, the total antioxidant capacity was evaluated according to the phosphomolybdate method. The extracts were produced using Soxhlet, ultrasound and rotary evaporator with aqueous solvent techniques. The results obtained revealed that the total phenolic content of the aqueous extracts of Turnera subulata in the different techniques (soxhlet = 135.7 ±0.1; ultrasound = 123.8 ±2.5 and rotary evaporator = 131.0 ±0, 1 mg/g) were