Caracterização da camada de reconhecimento de transdutores ópticos baseados no princípio da ressonância de plásmons de superfície.

Abstract

The use of surface plasmon resonance (SRP) as a bio-transducer requires the formation of a layer recognition on a metallic film. about metal a layer of proteins is formed on which antibodies must be deposited for the realization of an immunoassay. The protein is deposited non-homogeneous on the metallic surface by means of adsorption. A mathematical model was developed to simulate the entire process for the formation of this layerof protein (solution transport and adsorption) and the extinction of the SRP considering irregular layers and not homogeneous. The complexity of the model makes its analytical solution impossible, using the finite element method using software COMSOL Multiphysics. There was also developed a theory of equivalent means to represent a irregular and non-homogeneous layer as being regular and homogeneous. Im iterative method based onoptimization was created with this theory to characterize the recognition layer in a biotransducer a from your SRP response. The method was applied to treat data from adsorption experiments with neutrovidin, bovine albumin and hemoglobin obtaining results compatible with the literature.