ARAÚJO, A. G. P.; Araújo, Amanda Geovana Pereira de.; ARAÚJO, AMANDA GEOVANA PEREIRA.; http://lattes.cnpq.br/3946322725458190; ARAÚJO, Amanda Geovana Pereira de.
Resumen:
One of the challenges encountered all over the world is the increase in the types of antimicrobial
resistance, given that bacteria are everywhere and when pathogens infect infections in the
human host. In hospital environments, they cause irreversible repercussions, sometimes
uncontrollable by health agencies. The research aims to perform an analysis of hospital
microorganisms in hospital settings without bacteria screening, eliminating multicultural,
population screening, molecular and microbial bacterial screening. Methodologically, as
triplicate samples were collected at strategic points of the Hospital, later the cultivation of
strains, counting and isolation of strains, as well as antibacterial screening in different results
of Ampicillin. For sample analysis, techniques such as: DNA collection, Polymerase Chain
Reaction, Random Polymorphic DNA RAPD markers - and the 16S ribosomal gene were
performed. The results obtained with the degree of importance, the degree of cleanliness and
the identification of suitable characteristics, taking into account the high number of colonies
and the variability that demonstrates the great need for cleaning of local communities
Antibacterial screening with ampicillin showed significance, and as molecular techniques, as
the evaluations of studies of RAPD bacterial isolates if promissory. In summary, it is important
to be concerned with the hygiene of environments and clinical staff in the hospital environment,
especially in areas of high contact, to assist in the control of pathogenic bacteria and possible
future infections. The understanding of the microbial population present in these places is of
great scientific, social and economic importance, as it helps in decision-making and solutions
by the government.