Análise microbiológica de bactérias em ambiente hospitalar do município de Cuité - PB.

Abstract

One of the challenges encountered all over the world is the increase in the types of antimicrobial resistance, given that bacteria are everywhere and when pathogens infect infections in the human host. In hospital environments, they cause irreversible repercussions, sometimes uncontrollable by health agencies. The research aims to perform an analysis of hospital microorganisms in hospital settings without bacteria screening, eliminating multicultural, population screening, molecular and microbial bacterial screening. Methodologically, as triplicate samples were collected at strategic points of the Hospital, later the cultivation of strains, counting and isolation of strains, as well as antibacterial screening in different results of Ampicillin. For sample analysis, techniques such as: DNA collection, Polymerase Chain Reaction, Random Polymorphic DNA RAPD markers - and the 16S ribosomal gene were performed. The results obtained with the degree of importance, the degree of cleanliness and the identification of suitable characteristics, taking into account the high number of colonies and the variability that demonstrates the great need for cleaning of local communities Antibacterial screening with ampicillin showed significance, and as molecular techniques, as the evaluations of studies of RAPD bacterial isolates if promissory. In summary, it is important to be concerned with the hygiene of environments and clinical staff in the hospital environment, especially in areas of high contact, to assist in the control of pathogenic bacteria and possible future infections. The understanding of the microbial population present in these places is of great scientific, social and economic importance, as it helps in decision-making and solutions by the government.