RAMOS, B. F. G.; http://lattes.cnpq.br/5096142050213039; FORTE, M. P. N.; Forte, Morgana P. N.; FORTE, MORGANA PORDEUS DO NASCIMENTO.; http://lattes.cnpq.br/8913862437704521; RAMOS, Bibiana Ferreira Gouvêia.; FORTE, Morgana Pordeus do Nascimento.
Résumé:
Chitosan is a polymer derived from chitin, of low cost,
renewable and biodegradable, being of great importance
economic and environmental. Although in recent years, this compound
has been described as a potent antimicrobial, its action
antifungal remains little explored. This work aimed
evaluate the antifungal action of low, medium and high chitosans
molecular weight on different Candida species. To the
main strains of the genus Candida (ATCC) were investigated:
Candida sp ATCC 34147, C. albicans serotype A ATCC 36801 and
C. albicans serotype B ATCC 36802, C. glabrata ATCC 2001, C.
Krusei ATCC 34135 and Candida tropicalis ATCC 28707. They were
obtained by request to Fundação Oswaldo Cruz, through the
Minimum inhibitory concentration (MIC) in solid medium and in medium
liquid. The compound was tested at different weights against the
pure concentrations, 20mg/mL, and in the dilutions referring to the
concentrations of 10 mg/mL, 5 mg/mL, 1.75 mg/mL and 0.875 mg/mL.
It was observed that all strains tested were sensitive to at least
least one type of chitosan, thus demonstrating the character
fungistatic of the studied compound. In view of the results, it was
selected chitosan with greater antifungal action, as well as
the most sensitive strain, for detailed evaluation in
different growth times of the species, the result of which
MIC up to 24 hours was 0.875 mg/mL, the lowest value tested
in the study, and the Minimum Fungicide Concentration (MFC) was 1.75
mg/mL, while in the 144-hour growth the MIC was
5mg/mL and CFM of 20mg/mL. Such results demonstrated a
high power of fungicidal action of chitosan against a fungus
pathogen, being even more effective when compared to
to fluconazole.
