Prospecção do genes PR-5 em amburana cearensis (Allemão) A. C. Smith.

Abstract

Genes encoding F'R—5 proteins are important for biotechnological applications because they present antimicrobial activity and conter resistance to biotic and abiotic stresses. Genomic studies on Semiari—d plants are scarce, although the genes of the plants are evolutionarity adapted to the region and have great potential for use in plant molecular breeding programs. In this context. in this work was aimed to prospect PR—5 genes in & typical plant of the caatinga, Amburane cearensis (Allemão) A.C.Sm, weli known for its medicinal and wood use, but genetically little studied, by PCR using specific primers. For this, total DNA was puritied from from coumaru young leaves , using the CTAB extraction protocol; The amount and purity of DNA extracted were analyzed by spectrophotometer reading and agarose gel electrophoresis; and Coumaru DNA was amplified by the polymerase chain reaction (PCR) with different combinatio—ns of primers specific for genes encoding PP.-5 proteins. As results, the CTAB protocol was efficient for an extraction of DNA cumaru. The extracted coumaru DNA was amplifiable by PCR, generating fragments of expected length for PP.-5 genes. The expected in lenght amplicons for complete PRS-Eike genes need to be purified and sequenced, aiming to prove the presence of the genes, and it is the theme for the next steps of this work.