Avaliação do potencial antibacteriano e antiaderente do óleo essencial da Citrus sinensis contra cepas de Pseudomonas aeruginosa.

Abstract

Most dental infections occur through cross-contamination. P. aeruginosa is a bacterium that has humidity favorable to its development, either through the water present in dental systems, devices used in procedures, as well as pipes that are connected to the dental chair, thus being able to reach the oral cavity. Therefore, due to the bacterial resistance of this species developed against antimicrobials and disinfectant solutions, new means are sought to effectively combat these pathogens, in particular, natural products. Therefore, the aim of this study was to evaluate the antibacterial and antiadherent activity of Citrus sinensis essential oil against strains of Pseudomonas aeruginosa. Assays were performed in duplicate with a 96-hole plate, using microdilution techniques to determine the antibacterial potential through the Minimum Inhibitory Concentration (MIC) and the bactericidal activity through the Minimum Bactericidal Concentration (MBC), finally, the Concentration Minimum Inhibitory Adherence (MIAC), using the 30° inclined tube technique, in the presence of 5% sucrose. Thus, the essential oil of Citrus sinensis obtained a MIC of 1000 ug/mL against P. aeruginosa strains (PA104 and PA109) and CBM of 1000 mg/mL and >1000 ug/mL for strains PA104 and PA109, respectively. The CIMA of the essential oil against the strains under study was not able to inhibit adherence to the tube, when compared with the CIMA of 1:4 for the 0.12% Chlorhexidine Digluconate (positive control of the research). Thus, it is concluded that the essential oil of Orange showed moderate antimicrobial activity for both strains tested, it was bactericidal for strain PA104 and bacteriostatic for strain PA109, however it did not show antiadherent action for the strain of P. aeruginosa under study, in the methodology used.