LEITE, C. A.; http://lattes.cnpq.br/1652940079221996; LEITE, Clotildes Alvino.
Résumé:
Despite the wide use of cellulases, the high cost has made some expensive processes, studies
have been developed in order to produce this enzyme through a process of solid state
fermentation from lignocellulosic agroindustrial residues, thereby reducing the production
costs of enzyme, and adding value to waste. The objective of this study was to evaluate the
enzymatic production of cellulases by the filamentous fungus Trichoderma reesei LCB 48 by
solid state fermentation using sisal fiber as substrate. The characterization of sisal fiber was
performed to meet their physical composition, physico-chemical and chemical. The
characterization demonstrated that this substrate has the potential to be used in the
fermentation for production of cellulolytic enzymes, especially for introducing a satisfactory
alfacelulose percentage (58.40%), which is inducer of these enzymes and acid pH optimum
for the production of enzymes in solid state fermentation with fungi (4.35). Fermentation was
performed using the microorganism Trichoderma reesei LCB 48 with a concentration of 107
spores/g, at a temperature of 28 °C, substrate at 70, 80 and 90% humidity and
supplementation with the basic means of inducing enzyme activity of Mandels and Weber.
Samples were collected during the process to pH, moisture, reducing sugars and enzymatic
activity, expressed as carboxymethylcellulase, until a time of 474 hours. The higher enzyme
activity obtained for sisal fiber was 1.21 U/g in 450 hours of fermentation, in a substrate with
70% water content, a positive factor due to reduction of water use in the fermentation process.
