FERNANDES, Annielle Regina da Fonseca.; AZEVEDO, Sérgio Santos de.; PIMENTA, Carla Lauise Rodrigues Menezes.; UCHÔA, Iana Carolina Pordeus.; BRASIL, Arthur Willian de Lima.; MEDEIROS, Nayara da Silva.; MELO, Márcia Almeida de.; ALVES, Clebert José.; PIATTI, Rosa Maria.; PINHEIRO, Eliana S.; BATISTA, Carolina de Sousa Américo.; AZEVEDO, Adílio Santos de.; GENOVEZ, Margareth Elide.; TORRES, Leonardo Mendes.
Abstract:
The aim of this work was to determine the prevalence of anti-Brucella canis antibodies in dogs from
Patos municipality, Paraíba State, as well as to identify risk factors associated with seropositivity, and to
confirm the infection by microbiological culture and Polimerase Chain Reaction (PCR). Sera from 193
dogs were tested by the agar gel immunodiffusion test (AGID), using lipopolysaccharides and proteins
antigens from Brucella ovis, strain Reo 198, for the serological diagnosis of the infection. For the isolation
of Brucella canis blood and bone marrow cultures from seropositive animals were carried out. Six animals
tested seropositive in AGID, resulting in a seroprevalence of 3.11% (95% CI = 1.15% – 6.64%). Brucella
canis was isolated from bone marrow from one seropositive animal, with confirmation by PCR. Do not
clean the environment in which the animals stay was identified as risk factor (odds ratio = 7.91). The
present work was the first on reporting the Brucella canis isolation from dogs in the Northeast region of
Brazil.
