Avaliação de diferentes proporções de cálcio e fósforo na regeneração do tecido ósseo em defeito provocado na metáfise femoral de coelhos (Oryctolagus cuniculus). estudo clínico-cirúrgico, radiológico e histológico.

Abstract

The experiment was developed in the Veterinary Hospital of UFCG, campus of Patos, PB. Twenty rabbits of both sexes, weighing on average 2,5 kg, were purchased from a creation situated in the city of Patos – PB. The animals were divided randomly in two groups of 10 animals in accordance with period that they were euthanized (30 or 60 days after the surgical procedure). For comparative evaluation among them, four types of biomaterials graft with different preparations of Ca/P have been used. The animals were housed individually in hutches, fed a commercial dog food and given water ad libitum. They had a period of adaptation of seven days before the beginning of the experiment. Four implants, two in each femur, were used in each animal. The implants were situated in proximal and distal metaphysis. The biomaterial implants were placed in each hole carried out with a 2,0 mm trefina after the bone has been accessed surgically. In proximal metaphysis of the left pelvic member the CPP700 implant was used, in distal metaphysis of the left pelvic member a CPP900 implant was used, in proximal metaphysis of the right pelvic member TCP/HA implant was used, and in distal metaphysis of the right pelvic member HA was used, in each animal. After the introduction of the implants, the suture of the muscle, subcutaneous tissue and skin was carried out. The animals presented normal clinical evolution, without signs of infection, complication, or dehiscence of the wound and all surgical wounds had first intention healing. It did not have statististical difference in the radiographic evaluation in relation to the healing induction of influenced by the biomaterials between groups, however, it could be observed a trend of bone healing induction in the animals of group 60 days that have been used TCP/HA and HA implants. Histological evaluation has shown that TCP/HA and HA materials had, in such a way, provided a greater stimulation of bone healing in group 30 days and in the one of 60 days, although in the group 60 days the healing was more evident. The results gotten in this experiment allow to conclude that the biomaterials used revealed biocompatible and TCP/HA and HA implants provided induction in the bone healing faster than the others implants.