MAMEDE, Aline Guedes.; ALFARO, Carlos Henrique Peña.; SILVA, Allan Kariston Borges.; MOURA, Marquiliano Farias de.; TORRES, Vera Lúcia de Lima.
Résumé:
This work aimed to study the effectiveness of different solutions using hyposmotic semen from two rams of
the Santa Inês breed, diluted in water, coco powder and milk powder and cooled to 5 ºC. The solutions were
made hyposmotic sodium citrate and fructose, at concentrations of 150 mOsmol/L and 300 mOsmol/L. Ten
samples were made by breeding and semen immediately evaluated and submitted to the hyposmotic test
periods of 12, 24 and 48 hours after dilution. The results it was found in vitro sperm viability after dilution
using the two solvents, and their conservation satisfactory to 5 ºC in the first 24 h. Concluding that the
hyposmotic test was effective in evaluating the plasma membrane of spermatozoa in different periods.
