Extração e caracterização do pó de colágeno obtido a partir de resíduos (pele e nadadeiras) de tilápia.

Abstract

Beneficiation of tilapia produce by-products that are arranged in the environment without treatment. Production of collagen and hydrolyzates from these byproducts of fish processing has changed this scenario, due emulsifying properties, foaming agents, colloidal stabilizers, biodegradable films, microencapsulating agents with tendency to substitute synthetic material for natural with a bioactivity and biocompatibility for medical applications. In the acid-base extraction method, residues were treated using solutions of: NaCl (0.8M), and different concentrations of NaOH (0.05M; 0.1M; 0.2M) and acetic acid (0.05M), aiming at the decoupling of collagen. Calculations were made to determine the collagen yield relative to the raw material, obtaining a higher yield of 12.68% with the concentration 0.1 M of NaOH, in lower and higher concentrations, variation in yield occurred. Morphological characterization was performed by optical microscopy (OM) and scanning electron microscopy (SEM) and chemical characterization by X-ray Dispersive Energy (EDS) technique and Fourier Transform Infrared Spectroscopy (FTIR). In the results of MO and SEM were obtained micrometric details of structure, topography and surface of collagen particles, with morphological similarities with other natural polymers In the EDS analysis it was shown the percentages of the expected chemical elements typically for organic polymers. FTIR assay showed through bands characteristics reported in literature on chemical bonds forming clusters between such elements reported in the EDS.