Análise da produção da enzima invertase a partir da fermentação em estado sólido utilizando substratos não convencionais.

Abstract

Invertase can be known also as ꞵ-fructofuranosidase D, and hydrolase, that can be detected in a wide variety of eukaryote and prokaryote organism. In the industry, it is the main enzyme used in the hydrolysis of sucrose, and also in obtaining the invert sugar syrup. For this enzyme production, the most widely way of fermentation in scientific production is the solid state fermentation. In this context, this study aimed to analyze the performance of the solid state fermentation in the invertase enzyme production, using the filamentous fungus Aspergillus niger IOC 4003 as an agent, using two waste (bran and rice bran mesquite). The fermentation took place in 250 ml Erlenmeyer flasks containing the inoculated medium with the chosen substrate, where the whole process is kept under incubation at room temperatureRegarding the period of this process, the fermentation occurred in seven days, and every 24 hours were collected flasks of each residue. From the fermented product extracted from each flask by vacuum filtration the pH, substrate consumption and enzymatic activity were analyzed. It was observed that in both fermentations, using rice bran and algaroba meal, they presented levels of invertase activity significantly in the process and were directly proportional to the carbon and nitrogen consumption present in the medium by the microorganism throughout the fermentation. In this bias, the invertase production by solid state fermentation adds value to the waste that would be discarded, strengthening the whole concept of sustainability in fermentative processes and economic viability of the enzymatic production industry.