DIAS, Emanuele Cardoso.; DANTAS, Gerbeson Carlos Batista.; FARIAS, Henriqueta Monalisa.; BRITO, Analu Freitas de Souza.; SILVA, Caroline Targino Alves da.; GERVÁSIO, Karenine Lacerda.; VAZ, Michelle Rossana Ferreira.
Resumen:
Invertase can be known also as ꞵ-fructofuranosidase D, and hydrolase,
that can be detected in a wide variety of eukaryote and prokaryote organism. In the
industry, it is the main enzyme used in the hydrolysis of sucrose, and also in obtaining
the invert sugar syrup. For this enzyme production, the most widely way of fermentation
in scientific production is the solid state fermentation. In this context, this study aimed to
analyze the performance of the solid state fermentation in the invertase enzyme
production, using the filamentous fungus Aspergillus niger IOC 4003 as an agent, using
two waste (bran and rice bran mesquite). The fermentation took place in 250 ml
Erlenmeyer flasks containing the inoculated medium with the chosen substrate, where
the whole process is kept under incubation at room temperatureRegarding the period of
this process, the fermentation occurred in seven days, and every 24 hours were collected
flasks of each residue. From the fermented product extracted from each flask by vacuum
filtration the pH, substrate consumption and enzymatic activity were analyzed. It was
observed that in both fermentations, using rice bran and algaroba meal, they presented
levels of invertase activity significantly in the process and were directly proportional to
the carbon and nitrogen consumption present in the medium by the microorganism
throughout the fermentation. In this bias, the invertase production by solid state fermentation adds value to the waste that would be discarded, strengthening the whole
concept of sustainability in fermentative processes and economic viability of the
enzymatic production industry.