COELHO, Glauciane Danusa.; SOUSA, Jucilene Pereira de.; LIMA, Caio de Azevedo.; LINS, Simone Aparecida da Silva.
Résumé:
The objective of the present study was to prospect fungi with potential for
production of amylase from fungi isolated in the Paraiba (semi - arid region) and
deposited in the Fungi Collection of the Center for the Sustainable Development of the
Semi - arid / Federal University of Campina Grande (CDSA / UFCG) . This work
represents a strategy to know the enzymatic diversity of organisms in this biome, as well
as to apply them in future biotechnological processes. The analyzes were carried out
from 50 isolates of this collection, of which only 27 showed growth in BDA medium and
of these only 10 (ten) (37.04%) presented amylolytic activity. The determination of the
enzymatic production was carried out in solid medium composed of agar and soluble
starch, by means of the determination of the enzymatic index (IE). The highest IE values
were obtained from the fungal isolates denominated CDSA 09, 18, 23 and 24 with values
of 3.79 ± 0.5; 3.50 ± 0.5; 3.4 ± 0.4 and 3.5 ± 1.0, respectively. Fungal isolates CDSA 23
and CDSA 54 showed the highest growth rates, filling the entire Petri dish in 144 hours
(6 days). The prospection of enzymes from microorganisms isolated from the Caatinga
is of extreme value in order to reveal the microbial biodiversity of this region that is still
little explored.
