Produção de amilase e protease obtidas por Metarhizium anisopliae var. anisopliae através da fermentação em estado sólido.

Abstract

Amylolytic and proteolytic enzymes are widely used in various biotechnological processes. At present, there is a perspective for the use of solid state fermentative processes in the production of enzymes of industrial interest, using low cost substrates. Among these substrates, we highlight the algaroba, which was used in this study as a source of carbon and energy for fungus growth. Therefore, the present study aimed to delineate the culture conditions for the production and thermal stability of the amylolytic and proteolytic enzymes obtained by Metarhizium anisopliae through the solid state fermentation in the algaroba fiber. The substrate used was characterized and 30 g of algaroba fiber, 0.3 ml of the conidial suspension (1 x 108 conidia/mL), humidity of 70 ± 10 %, temperature (T = 29 ± 1 °C). The amylolytic activity was determined by the DNS method. The thermostability was evaluated at a temperature range of 40° to 70 °C for 30 minutes. In the characterization were obtained pH 5.4, apparent density 0.23 g/mL, 19.12 mg/mL of ART, 12.33Â °brix of soluble solids, 48.65 % of humidity and 13.60 mg/mL of proteins. The crude extracts of the fermentation process showed amylolytic activity of 6.627 U/g at 72 hours of production and proteolytic of 175.8 U/g at 240 hours. The amylases showed a better enzymatic activity at the 30 °C production temperature. The results obtained demonstrated the potential of algaroba as an alternative culture medium for the production of enzymes of industrial interest.