GERVÁSIO, Debora Karenine Lacerda.; LIMA, Melina Kehtle Lins de.; DIAS, Emanuele Cardoso.; ANDRADE, Adrielly Silva Albuquerque de.; SILVA, Caroline Targino Alves da.; SOUSA, Adna Cristina Barbosa de.; SOUSA, Carlos Alberto Bispo de.; ALMEIDA, Andréa Farias de.
Resumen:
The use of agroindustrial residues can be an alternative for the production
of microbial enzymes at low cost, besides providing a more noble destination of these
residues, thus avoiding the accumulation in the environment. For this purpose, solid state
fermentation processes are considered, as well as the applicability of the enzymes
produced in these systems in the industry. The objective of the present work was to
evaluate the production of cellulolytic and proteolytic enzymes, using solid state
fermentation, using the green coconut bagasse as substrate by the fungus Aspergillus
sp. The characterization of the substrate was carried out, as well as a factorial
experimental design 22, in order to analyze the influence of moisture and the addition of
nitrogen source (ammonium sulphate). The levels of the independent variables applied
were: 60%, 70% and 80% humidity and 0%, 0.5% and 1% (w/v) ammonium sulfate.
Considering the result of the design, the CMCase activity was positively influenced by the lower level of the ammonium sulphate concentration and the higher level of humidity.
For FPase activity, the higher levels of both ammonium sulphate concentration and
moisture favored the enzymatic production. As for the proteases, the lower levels of both
ammonium sulphate concentration and moisture favor the production of these enzymes.
Therefore, the concentration of ammonium sulphate and moisture applications on design
optimized the enzymatic production process from the green coconut residue.