Produção de enzimas por Aspergillus sp. utilizando o resíduo de coco verde como substrato.

Abstract

The use of agroindustrial residues can be an alternative for the production of microbial enzymes at low cost, besides providing a more noble destination of these residues, thus avoiding the accumulation in the environment. For this purpose, solid state fermentation processes are considered, as well as the applicability of the enzymes produced in these systems in the industry. The objective of the present work was to evaluate the production of cellulolytic and proteolytic enzymes, using solid state fermentation, using the green coconut bagasse as substrate by the fungus Aspergillus sp. The characterization of the substrate was carried out, as well as a factorial experimental design 22, in order to analyze the influence of moisture and the addition of nitrogen source (ammonium sulphate). The levels of the independent variables applied were: 60%, 70% and 80% humidity and 0%, 0.5% and 1% (w/v) ammonium sulfate. Considering the result of the design, the CMCase activity was positively influenced by the lower level of the ammonium sulphate concentration and the higher level of humidity. For FPase activity, the higher levels of both ammonium sulphate concentration and moisture favored the enzymatic production. As for the proteases, the lower levels of both ammonium sulphate concentration and moisture favor the production of these enzymes. Therefore, the concentration of ammonium sulphate and moisture applications on design optimized the enzymatic production process from the green coconut residue.