COELHO, Glauciane Danusa.; FREITAS, Caroline da Silva.; BARBOSA, Eliel Gomes.; BELÉM, George Lacerda.; SILVA, Lucas Rodolfo Inácio.; LEAL, Maxsuel da Costa.; RODRIGUES, Ravenna Lins Rodrigues.; NASCIMENTO, Ana Verônica Silva do.
Abstract:
The agroindustrial activities have promoted an increase in the production
of ligninolytic residues. Among the products of agroindustrial origin, it is possible to
emphasize the green coconut bagasse, which has generated large amounts of residues
due to increase of the green coconut processing for coconut water commercialization.
The need for sustainable development has demonstrated the need for cleaner
production. Thus, in this work the bagasse of the green coconut was used for the
production of cellulolytic enzyme (CMCase), from a fungus isolated from the thermophilic
phase of a composting process. The effect of temperature and humidity on the kinetics
of CMCase production was evaluated in solid state fermentation using factorial design
22. The fungus used was identified as Aspergillus sp. and was able to colonize the green
coconut bagasse and produce CMCase, and the best CMCase production was obtained
at 45 ° C and 45% humidity (bu). The CMCase obtained in this condition presented
optimum temperature and pH of 50 ° C and 5.0, respectively, and the half-life was
estimated to be 17.5 minutes.