CAVALCANTI, Rayza Morganna Farias.; GUIMARÃES, Luís Henrique Souza.
Resumen:
Tannases (tannin acyl hydrolase, EC 3.1.1.20) are enzymes of great
biotechnological interest, with potential for application in different industrial sectors such
as food, chemical, beverage and pharmaceutical industries. These enzymes act in the
hydrolysis of hydrolysable tannins, releasing glucose and gallic acid from gallotannins.
In this context, filamentous fungi, especially endophytic fungi, have been highlighted as
enzymatic sources. Therefore, the objective of this study was the production of tannase
by the endophytic fungus Aspergillus niger ANG18, isolated from bark of angico
(Anadenanthera colubrina Vell), under Solid-State Fermentation (SSF), using
agroindustrial residues and agroforestry products as substrates, selecting the best
conditions for maximum enzymatic production, as well as the determination of some
physical-chemical properties of the enzyme. The endophytic fungus A. niger ANG18
presented higher enzymatic production (29.82 U/g of substrate) when cultivated under
SSF using eucalyptus (Eucalyptus ssp.) leaves as substrate/carbon source moistened
with 1:1 (m/v) Khanna salts for 96 h at 37°C and 60% relative humidity. The tanase
contained in the crude filtrate presented temperature and apparent optimum pH of
activity between 40 and 45ºC, and pH 5.0, respectively, being slightly activated (16.93%)
in the presence of BaCl2.In this way, A. niger ANG18 can be considered as an alternative
producer of tannases in SSF, being this the first report of production of tannase by an
endophytic fungus in SSF.